An efficient approach to eliminate steryl ethers and miscellaneousesters/ketones for gas chromatographic analysis of alkenonesand alkenoates
发布时间：2024-01-08
元数据
名 称
An efficient approach to eliminate steryl ethers and miscellaneousesters/ketones for gas chromatographic analysis of alkenonesand alkenoates
科技资源标识
CSTR:11738.14.NCDC.XDA14.PP4416.2024
DOI
10.1016/j.chroma.2019.02.064
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摘 要
Long-chain alkenones (LCAs) and alkenoates (LCEs) are highly valuable biomarkers for paleotemperature reconstructions. A major problem, however, for accurate quantification of these compounds using gas chromatography (GC) is co-elution with steryl ethers, wax esters, saturated ketones and other numerous mid-polarity compounds frequently encountered in marginal marine and lake sediments. Co-elution dur_x0002_ing GC separation is prevalent, particularly ifthe full homologous series of alkenones and alkenoates are to be analyzed. Taking advantage ofthe presence oftwo or more double bonds in LCAs and LCEs,the conventional silica gelimpregnated withsilvernitratehas previously beenused to remove co-eluting compounds for LCAs. However,this conventional argentation chromatography is hampered by the extreme instability of silver nitrate, poor reproducibility, low recovery and short lifetime. Here we demonstrate a highly efficient flash chromatographic approach based on silver thiolate chromatographic material (AgTCM) that overcomes the shortcomings of the traditional argentation chromatography and allows repeated sample preparation (up to 62 samples in one test) with little loss in separation efficiency. AgTCM selectively extracts LCAs and LCEs and effectively eliminates co-eluting compounds including steryl ethers and wax esters for the subsequent gas chromatography (GC) analysis. This new method,therefore, allows low-cost and high-throughput sample preparation for comprehensive quantification of the full homologous series of LCAs and LCEs in marine and lake sediments
Li Wang. An efficient approach to eliminate steryl ethers and miscellaneousesters/ketones for gas chromatographic analysis of alkenonesand alkenoates. 国家冰川冻土沙漠科学数据中心(http://www.ncdc.ac.cn), 2024. https://cstr.cn/CSTR:11738.14.NCDC.XDA14.PP4416.2024.
Li Wang. An efficient approach to eliminate steryl ethers and miscellaneousesters/ketones for gas chromatographic analysis of alkenonesand alkenoates. 国家冰川冻土沙漠科学数据中心(http://www.ncdc.ac.cn), 2024. https://www.doi.org/10.1016/j.chroma.2019.02.064.
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CSTR:11738.14.NCDC.XDA14.PP4416.2024
10.1016/j.chroma.2019.02.064